FastQCFastQC Report
Mon 6 Jun 2016
ERR1378874.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378874.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724733
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32310.44581935692179053Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAGTTATCTCGTATGCC25610.35337151751058665Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAGTTATCTCGTATGCCGTC23630.3260511112368279Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2750.036.81422843
TGCCGTC2850.035.52250345
ATGCCGT2950.034.3183544
ATCGGAA4650.031.9276527
GATCGGA4950.029.99264126
TCGGAAG5050.029.84622428
AGATCGG5100.029.5536125
CCGAGAT5500.027.40425922
CGAGATC5650.026.6767123
TCGTATG5950.024.19908143
GAGATCG6150.024.14208424
CGTATGC5800.024.04914344
TATCTCG5900.024.02284639
AATGCCG10600.023.97996318
TCTCGTA5950.023.8209741
GACCGTC5950.023.81932829
CGTCAGT5950.023.44609632
TGCCGAG10750.023.43772520
CTCGTAT6050.023.42723742
CCGTCAG6100.023.23841931
Produced by FastQC (version 0.11.5)