FastQCFastQC Report
Mon 6 Jun 2016
ERR1378872.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378872.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031434
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT42480.41185378802715444Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCCGTC32710.31713129487684133Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCC27170.26341966621228313Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3850.037.39699643
ATGCCGT4050.035.55023244
TGCCGTC4200.034.81621645
GATCGGA5900.033.55429526
ATCGGAA6100.032.08535827
TCGGAAG6400.031.98894728
AGATCGG6300.031.78095425
AATGCCG13400.026.35799218
GAGATCG7600.026.34473824
TGCCGAG13700.026.2734420
CCGAGAT7700.026.002622
ATGCCGA13600.025.97037519
CGAGATC7800.025.66923123
GAATGCC15350.023.00958417
GCCGAGA15600.022.20821221
GGAATGC16300.021.94456516
TCTCGTA7000.020.88972938
GCACCCG654.242109E-420.7661212
GTATGCC7100.020.59550742
CTCGTAT7150.020.45148339
Produced by FastQC (version 0.11.5)