FastQCFastQC Report
Mon 6 Jun 2016
ERR1378871.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378871.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660087
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT46380.7026346527048707Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCTTATCTCGTATGCCGTC36620.5547753553698225Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCTTATCTCGTATGCC25580.38752467477771874Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3900.040.38128743
ATGCCGT4150.037.4065544
TGCCGTC4350.036.2039145
GATCGGA5950.033.6501426
ATCGGAA6200.033.0189727
TCGGAAG6450.032.43918628
AGATCGG6550.030.56999825
CCGAGAT6700.030.2213922
CGAGATC6650.030.11029623
GAGATCG7200.027.81020524
AATGCCG13600.026.7972318
ATGCCGA13800.026.41086619
TGCCGAG13900.026.22085820
GAATGCC14550.025.35681217
GCCGAGA14300.025.17274921
CTCGTAT6550.023.70033539
CCGGATC6700.023.50552628
GGAATGC15950.023.41322316
CGGAAGA8950.023.3779629
TCGTATG6650.023.34393740
Produced by FastQC (version 0.11.5)