FastQCFastQC Report
Mon 6 Jun 2016
ERR1378870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738009
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32470.4399675342712623Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCTAATCTCGTATGCCGTC21030.28495587452185545Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCTAATCTCGTATGCC16510.22371000895653032Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.037.35927643
GATCGGA4550.034.1123526
ATGCCGT3050.033.19742244
ATCGGAA4700.033.02365527
TCGGAAG5050.032.96652228
TGCCGTC3050.032.459745
AGATCGG5050.030.7348925
TTACGCC300.00515237129.99250423
AATGCCG9450.028.32625418
ATGCCGA9550.027.794119
CCGAGAT5600.027.71628422
TGCCGAG9750.027.22396520
GAGATCG5800.026.76055324
CGAGATC5950.026.08591523
GAATGCC10250.025.89596617
ACCGTAC4100.024.6889527
CGTACCT4150.024.39479629
GCCGAGA10550.024.30671921
CCGTACC4200.024.10438328
CGGAAGA6950.023.95409229
Produced by FastQC (version 0.11.5)