FastQCFastQC Report
Mon 6 Jun 2016
ERR1378868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999404
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT23260.23273871227251441Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACTGATCTCGTATGCC18250.18260883486557988Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACTGATCTCGTATGCCGTC18200.18210853668786597Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.036.62493543
GCGTATA250.002108334335.99527721
TGCCGTC2300.034.2363545
ATGCCGT2500.032.3973744
GATCGGA3350.031.56144326
ATCGGAA3450.030.6466227
AGATCGG3600.030.62098525
CCGTTCA300.005151503729.99456418
TCGGAAG3950.027.33955228
AATGCCG7900.024.48923118
ATGCCGA8400.023.30051419
TGCCGAG8450.022.89640420
CTCGTAT3950.021.64381242
TCTCGTA4000.021.37326641
ATCTCGT4050.021.10939840
CGTATGC4050.021.10939844
AGACCGT4300.020.92853428
ACCGTTA4200.020.89325130
TCGTATG4100.020.85196543
CCGTTAC4150.020.60589231
Produced by FastQC (version 0.11.5)