FastQCFastQC Report
Mon 6 Jun 2016
ERR1378864.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378864.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981687
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT49540.5046414997855732Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGATTATCTCGTATGCCGTC37240.3793469812679601Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGATTATCTCGTATGCC33830.34461085865454055Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4350.038.27408643
TGCCGTC4800.035.15461745
ATGCCGT4900.033.97801644
ATCGGAA6050.030.86161827
AGATCGG6200.030.47935325
TCGGAAG6500.030.45857228
GATCGGA6150.030.35980226
GAGATCG7000.026.99599624
CCGAGAT7250.026.37539922
AATGCCG15000.024.74506818
CGAGATC7700.024.54181523
ATGCCGA15150.024.35282519
TGCCGAG15400.023.81140320
GAATGCC16500.022.3591817
GCCGAGA16000.022.3560621
CTCGTAT7750.021.48287439
TCTCGTA7900.021.0717538
CGTATGC7950.020.94242341
CCGCTGA8250.020.72525428
TATCTCG8050.020.68121536
Produced by FastQC (version 0.11.5)