FastQCFastQC Report
Mon 6 Jun 2016
ERR1378862.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378862.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065148
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT38860.36483192945956805Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTATGCCGTC29410.2761118642667498Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTATGCC25510.239497234187174Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3050.037.6172243
TCGGAAG6600.033.4055728
ATCGGAA6450.032.4369227
GATCGGA6550.031.941726
ATGCCGT3750.031.79515644
AGATCGG6750.030.99527725
TGCCGTC3950.029.61574245
CCGAGAT7950.026.88269622
GAGATCG8300.025.20700524
AATGCCG13450.025.08911318
CGGTAAG450.001227585224.99619342
GTCGGAA450.001227585224.99619327
ATGCCGA13600.024.81239719
CGAGATC8450.024.75954223
TGCCGAG13750.024.54171420
GAATGCC14550.023.34695817
GCCGAGA14450.022.88578621
CGGAAGA9850.022.38342929
CTCGTAT5600.021.69312342
CGTATGC5650.021.50114844
Produced by FastQC (version 0.11.5)