FastQCFastQC Report
Mon 6 Jun 2016
ERR1378861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955335
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT28400.29727791821716987Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACATTATCTCGTATGCC20180.2112348024514961Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCACATTATCTCGTATGCCGTC19080.19972051688674652Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2350.038.29707343
ATCGGAA4650.031.4443727
ATGCCGT2900.031.03383344
TCGGAAG4950.030.90359128
GATCGGA4850.030.14769426
TGCCGTC3150.029.99936945
AGATCGG5100.028.67286725
AATGCCG9200.026.65146818
ATGCCGA9400.026.08714719
CCGAGAT5700.025.65467322
TGCCGAG9650.025.17818520
CGAGATC5950.024.57674623
GAGATCG6050.024.17051524
CGGAAGA6700.022.83175729
TATCTCG4100.022.4995336
TCTCGTA4100.022.4948238
GAATGCC10900.022.49481817
ATCTCGT4200.021.96382537
GCCGAGA10800.021.8722521
CTCGTAT4250.021.7054339
Produced by FastQC (version 0.11.5)