FastQCFastQC Report
Mon 6 Jun 2016
ERR1378860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722709
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT22690.3139576233311056Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAATTATCTCGTATGCC16770.2320436026118396Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCAATTATCTCGTATGCCGTC16160.22360313763907744Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2400.038.43507443
ATGCCGT2500.035.9977344
TGCCGTC2500.035.9977345
AGATCGG4000.034.30558825
GATCGGA4000.034.30558826
ATCGGAA4000.034.30558827
TCGGAAG4050.033.8820628
CCGAGAT4600.029.83094622
AATGCCG7700.029.79919218
TGCCGAG8100.028.60534720
ATGCCGA8200.028.25650419
GGAATGC8400.027.04812216
GAATGCC8700.025.8568617
GCCGAGA9000.025.74481421
CGAGATC5600.024.50399423
GAGATCG5750.023.86475824
TCTCGTA4000.023.05785638
CGGAAGA5900.022.87674729
CTCGTAT3950.022.78337339
TATCTCG3950.022.78179736
Produced by FastQC (version 0.11.5)