FastQCFastQC Report
Mon 6 Jun 2016
ERR1378857.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378857.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072022
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT43270.40362977625459184Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCCGTC31250.2915052116467759Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCC29040.2708899630791159Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3550.038.02245343
ATGCCGT3650.037.59708844
TGCCGTC4000.033.74492645
ATCGGAA6250.032.39361627
TCGGAAG6600.031.3574628
GATCGGA6700.030.55368226
AGATCGG6750.030.3273625
GAGATCG7700.026.58567424
CCGAGAT8100.026.38369222
AATGCCG13850.025.66281318
ATGCCGA13900.025.40866519
CGAGATC8350.025.05494523
TGCCGAG14050.024.97728720
GCCGAGA15300.022.64259721
GAATGCC15750.022.42415417
AACACGG955.5414966E-721.31159221
TAACACG855.0402778E-621.172320
CGGAAGA9950.021.0269929
GGAATGC16700.021.01382416
CCGTTGT6650.020.9742931
Produced by FastQC (version 0.11.5)