FastQCFastQC Report
Mon 6 Jun 2016
ERR1378856.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378856.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786629
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT38020.4833282271566393Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCCGTC30280.3849336853840883Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCC27840.35391525102685Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4100.038.95857243
ATGCCGT4200.037.4953444
TGCCGTC4300.036.62335645
GATCGGA5350.035.74094826
TCGGAAG5550.034.86274728
ATCGGAA5600.033.7436627
AGATCGG5800.032.97213725
CGAGATC6600.028.97551323
GAGATCG6650.028.75765224
AATGCCG12400.028.66396918
CCGAGAT6700.028.20724122
TGCCGAG12750.027.704220
ATGCCGA12900.027.38205719
GAATGCC13350.026.6242117
GCCGAGA13500.025.66510821
TCTCGTA6250.025.55519938
TATCTCG6250.025.1984736
CTCGTAT6250.025.19686739
GACCGTT6350.025.15275426
ACCGTTC6350.025.15275427
Produced by FastQC (version 0.11.5)