FastQCFastQC Report
Mon 6 Jun 2016
ERR1378855.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378855.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences761838
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35560.4667659003620192Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTCAATCTCGTATGCCGTC28790.3779018636508024Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTCAATCTCGTATGCC28000.36753220500946393Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3450.038.47399543
TGCCGTC3650.036.982245
ATGCCGT3600.036.8709144
GATCGGA5250.035.5651526
ATCGGAA5450.034.26000627
AGATCGG5600.034.14825
TCGGAAG5950.032.13929428
CCGAGAT6500.029.7659322
CGAGATC6900.028.0403723
GAGATCG7100.027.250524
AATGCCG12350.026.77665118
TGCCGAG12750.026.11475220
ATGCCGA12800.025.8369819
GCCGAGA13300.024.86566421
GAATGCC13650.024.22649217
GGAATGC14000.023.78151716
AGGAATG15350.021.54342815
AGACCGT6750.021.33096928
CGGAAGA9150.020.8993229
CGTGTTC6850.020.69113532
Produced by FastQC (version 0.11.5)