FastQCFastQC Report
Mon 6 Jun 2016
ERR1378854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931906
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT27360.2935918429541177Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATCTATCTCGTATGCC22110.23725568887849202Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATCTATCTCGTATGCCGTC19930.21386277156708938Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.038.7168743
TGCCGTC2500.033.2965145
TCGGAAG4750.033.15441528
ATCGGAA4550.032.13603627
AGATCGG4500.031.99664325
GATCGGA4750.030.7829426
ATGCCGT2750.030.2695544
AATGCCG9150.025.56834418
ATGCCGA9350.025.02411319
TGCCGAG9400.024.89100320
CGAGATC5800.024.82498223
CGTATGC4600.024.45395744
TCTCGTA4700.024.4123341
CCGAGAT5950.024.1991422
AGACCGC4950.024.0883828
CTCGTAT4800.023.90374442
GAGATCG6150.023.77799224
GACCGCA5150.023.59228929
TATCTCG5050.023.16588639
CCGCAAT5200.022.93274531
Produced by FastQC (version 0.11.5)