FastQCFastQC Report
Mon 6 Jun 2016
ERR1378853.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378853.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972582
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT32560.33477896979380656Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACCTATCTCGTATGCCGTC26080.26815219693557973Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAACCTATCTCGTATGCC18890.19422526840924467Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3350.036.93641743
ATGCCGT3600.033.74645244
TGCCGTC3650.033.28417245
ATCGGAA4050.030.54920427
GATCGGA4350.028.44236226
AGATCGG4850.026.90162325
CGTAACC4800.026.71593929
AATGCCG9600.026.47886718
TCGGAAG4800.026.2458928
GACCGTA4850.025.97398226
ACCGTAA4900.025.70893927
CCGTAAC4950.025.45056228
CTCGTAT4900.025.25244939
TATCTCG4950.024.99994336
CGTATGC4950.024.99737241
TCGTATG5000.024.74739640
TCTCGTA5100.024.2596638
AGACCGT5200.024.22572925
ATGCCGA10650.023.86827519
ATCTCGT5400.023.74994537
Produced by FastQC (version 0.11.5)