FastQCFastQC Report
Mon 6 Jun 2016
ERR1378852.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378852.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975262
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT19980.20486802520758526Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTACAATCTCGTATGCCGTC18620.19092305452278463Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTACAATCTCGTATGCC10060.10315176844786324Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2400.033.7472343
GATCGGA3300.031.3562426
ATCGGAA3400.031.09560427
ATGCCGT2750.031.08835644
TCGGAAG3500.030.21180728
TGCCGTC2850.029.99754145
AGATCGG3600.028.7476425
AATGCCG6750.028.32665318
ATGCCGA6850.027.91741619
TCGTATG3000.026.99778440
CGCTTAC3100.026.85263629
TGCCGAG7250.026.68746620
CCGCTTA3150.026.42640328
CGTATGC3100.026.12688841
CTCGTAT3050.025.81755439
GAATGCC7600.025.15853917
TCTCGTA3250.024.91975638
ACCGCTT3550.023.44517327
GTATGCC3600.023.12310242
GACCGCT3650.022.80284126
Produced by FastQC (version 0.11.5)