FastQCFastQC Report
Mon 6 Jun 2016
ERR1378851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745787
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT41950.5624930442606267Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGACTATCTCGTATGCCGTC32680.43819481970053115Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGACTATCTCGTATGCC27980.37517414489659917Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4150.037.94547343
ATGCCGT4450.035.89288744
TGCCGTC4450.035.89288745
GATCGGA4900.034.4330326
ATCGGAA4950.034.0852227
TCGGAAG5350.031.95728128
AGATCGG5350.031.5367925
CCGAGAT6000.029.24511722
GAGATCG6150.027.43444624
CGAGATC6400.026.36278723
TGCCGAG13100.025.58733220
ATGCCGA13150.025.49004219
AATGCCG13200.025.3934918
GAATGCC13900.024.11468117
GCCGAGA14500.022.96168521
GGAATGC15250.022.42248716
GACCGTT7600.021.01622826
AGGAATG16500.020.99649415
CTCGTAT7550.020.85744739
CCGTCAG654.241082E-420.76576415
Produced by FastQC (version 0.11.5)