FastQCFastQC Report
Mon 6 Jun 2016
ERR1378846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229685
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT31290.2544554093121409Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTACATCTCGTATGCC28820.23436896440958455Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGACTTACATCTCGTATGCCGTC23050.18744637854409868Illumina Paired End PCR Primer 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2850.034.7343343
ATCGGAA4950.033.6298327
AGATCGG5150.032.76195525
GATCGGA5150.032.76062426
ATGCCGT3150.031.426344
TCGGAAG5650.030.26083628
TGCCGTC3550.028.51906645
CGAGATC6450.025.80998823
CCGAGAT6650.025.03374922
AATGCCG11250.023.99533718
ATGCCGA11450.023.97011419
CTCGTAT5700.022.8930842
CGACTTA6150.022.31455232
TCTCGTA5850.022.3060841
TGCCGAG12250.022.22107520
CGTATGC6000.021.74842844
GACCGAC6200.021.40829
ACCGACT6200.021.40830
TCGTATG6100.021.39189543
GAGATCG7900.021.07271224
Produced by FastQC (version 0.11.5)