FastQCFastQC Report
Mon 6 Jun 2016
ERR1378844.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378844.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029435
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT35470.3445579371208478Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCATATATCTCGTATGCCGTC25280.24557159995531527Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCATATATCTCGTATGCC22250.21613797859991163Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2800.037.76251643
ATGCCGT3100.034.10807844
ATCGGAA5950.033.64743427
GATCGGA6000.033.3670426
AGATCGG6150.032.9205725
TCGGAAG6500.031.15095528
ACGTTAT300.00515301929.99284613
CCGAGAT7250.028.85658522
TGCCGTC3750.028.19601445
GAGATCG7450.027.47824
CGAGATC7750.026.99487123
TGCCGAG11900.026.65459820
AATGCCG11850.026.57593718
ATGCCGA11950.026.35482419
GCCGAGA12850.024.33389921
GAATGCC13500.023.82764817
CGGAAGA8750.023.39782729
GGAATGC14050.022.57468416
GAGCGGT15450.020.5570413
TCGTATG5150.020.53107640
Produced by FastQC (version 0.11.5)