FastQCFastQC Report
Mon 6 Jun 2016
ERR1378843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064273
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT40060.37640718124015177Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTGATATCTCGTATGCCGTC33890.3184333343042622Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTGATATCTCGTATGCC27000.253694305878285Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.037.14724343
ATGCCGT4400.036.3029944
TGCCGTC5050.031.63032745
ATCGGAA5700.029.5979727
GATCGGA5750.029.34059726
TCGGAAG5800.029.09175928
AGATCGG6100.027.65971825
AATGCCG13000.024.74390218
ATGCCGA13300.024.18804219
TGCCGAG13450.024.08554620
CGTATGC6950.022.98318741
CTCGTAT6950.022.98318739
TCGTATG6900.022.8236840
CCGAGAT7300.022.8047422
GAGATCG7350.022.64960724
GTATGCC7100.022.18075842
TATCTCG7250.022.03319436
TCTCGTA7200.021.87166438
GACCGCT7100.021.86080726
GCCGAGA14700.021.73137921
Produced by FastQC (version 0.11.5)