FastQCFastQC Report
Mon 6 Jun 2016
ERR1378842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818591
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT24730.30210446975351546Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTCATATCTCGTATGCCGTC18380.22453215341971755Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTCATATCTCGTATGCC16770.20486421179807743Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2450.035.81360643
TGCCGTC2650.033.11068745
ATGCCGT2750.032.72478544
GATCGGA3600.031.24380726
ATCGGAA3700.030.3993827
TCGGAAG4150.028.73128
AGATCGG4000.026.99629825
CCGAGAT4000.026.99629822
AATGCCG8050.025.42974318
CGAGATC4450.024.77188523
TGCCGAG8500.024.08493220
ATGCCGA8550.023.94408619
GAGATCG4750.022.73372524
CGGAAGA5350.022.28666329
GAGCGGT10000.021.618173
GAATGCC9500.021.5483617
GCCGAGA9250.021.40246821
GACCGGT4100.021.39819726
GCGGTTC9900.021.3776825
AGCGGTT10050.021.2865454
Produced by FastQC (version 0.11.5)