FastQCFastQC Report
Mon 6 Jun 2016
ERR1378840.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378840.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886049
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT31560.35618797606001473Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTCAAATCTCGTATGCC24510.27662127038120915Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTCAAATCTCGTATGCCGTC24210.2732354531182813Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.039.0523443
AGATCGG4750.034.10154325
TGCCGTC3200.033.0432745
GATCGGA4950.032.723726
ATGCCGT3200.032.3402244
ATCGGAA5100.031.76124227
TCGGAAG5200.031.5830928
CCGAGAT5950.027.9801422
AATGCCG10650.025.77038218
TGCCGAG10700.025.2308920
GAGATCG6500.024.92035724
ATGCCGA10950.024.65484219
CGAGATC6650.024.35824423
TGAACCG851.8956598E-723.81958410
GCCGAGA11750.022.7847521
ACCGCTC702.7055474E-522.49627513
GGAATGC12350.022.2230416
CTCGTAT5500.022.088542
GAATGCC12650.021.87384817
TCGTATG5600.021.69406143
Produced by FastQC (version 0.11.5)