FastQCFastQC Report
Mon 6 Jun 2016
ERR1378838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254079
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT31770.25333332270136094Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACACATCTCGTATGCC28410.2265407522173643Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACACATCTCGTATGCCGTC24610.19623963083665383Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2750.040.0852643
TGCCGTC3450.031.29993645
ATGCCGT3600.030.62068744
AGATCGG4400.028.12103825
ATCGGAA4450.026.7929127
GATCGGA4650.026.60809326
TCGGAAG4750.025.57534428
CCGTTAC5700.022.49862531
CGTATGC5700.022.4968344
CGTTACA5750.022.30298232
TCTCGTA5750.022.30120741
ACCGTTA5800.022.10895330
ATGCCGA10650.021.96873719
AATGCCG10650.021.96698418
CTCGTAT5900.021.73422642
TGCCGAG10800.021.66361420
TCGTATG5950.021.55158443
GACCGTT6000.021.37198829
CCGAGAT5850.021.15086622
CGAGATC6000.020.99704223
Produced by FastQC (version 0.11.5)