FastQCFastQC Report
Mon 6 Jun 2016
ERR1378837.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378837.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019393
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT31530.30930171190110195Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCTAATATCTCGTATGCC27130.26613877081753556Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCCTAATATCTCGTATGCCGTC24970.2449496906492393Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3550.038.025143
ATGCCGT3700.037.09160244
TGCCGTC4300.032.43924345
AGATCGG4650.028.0622525
GATCGGA4800.026.71397426
ATCGGAA4900.026.1687927
TCGGAAG5300.025.47089428
ATGCCGA11150.024.21329319
AATGCCG11150.024.21091718
TGCCGAG11150.024.01151520
CGCCTAA6050.022.68634829
CTCGTAT6100.022.49818639
GAATGCC12250.022.22051617
CCGCCTA6200.022.13639628
TCTCGTA6350.021.96673238
TCGTATG6350.021.96673240
CGTATGC6150.021.94944841
CCGAGAT5800.021.72238522
GCCGAGA12400.021.22812721
ATCTCGT6600.021.13569537
Produced by FastQC (version 0.11.5)