FastQCFastQC Report
Mon 6 Jun 2016
ERR1378830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882529
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGACCAAATCTCGTATGCCGTC14920.16905960030775194Illumina Paired End PCR Primer 2 (96% over 30bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT9410.10662539134691325Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGACCAAATCTCGTATGCC9010.10209296238423893Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1850.034.05002243
ATGCCGT1900.033.1539744
TGCCGTC2050.030.72807145
AGATCGG2050.024.14348425
ACCGGAC2650.023.77077127
GATCGGA2200.023.51994326
ATCTCGT2700.023.33057237
CTCGTAT2700.023.33057239
AGACCGG2700.023.33057225
TCTCGTA2800.023.30081438
GACCGGA2750.022.90638226
CGGACCA2750.022.90638229
CGTATGC2800.022.49733741
TCGTATG2800.022.49733740
CCGGACC3000.021.74742528
GAGACCG2900.021.72156724
AATGCCG5350.021.4460618
ATGCCGA5450.021.05255319
AATCTCG3050.020.65329436
ATCGGAA2400.020.62255927
Produced by FastQC (version 0.11.5)