FastQCFastQC Report
Mon 6 Jun 2016
ERR1378829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989231
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGGACATATCTCGTATGCCGTC13440.13586310982975663Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1550.039.191743
TGCCGTC1650.036.81644445
ATGCCGT1800.034.9983544
TACCGCA300.005141745330.0061683
GATCGGA1459.094947E-1221.72311426
TCTCGTA2800.021.69540638
CGTATGC2850.021.31478341
TATCTCG2850.021.31478336
AGACCGG2850.021.31478325
TCGTATG2850.021.31478340
CTCGTAT2900.020.94728939
GACCGGG2900.020.94728926
ACCGGGA3100.020.3226527
ATCTCGT3000.020.24904437
ATGCCGA4500.019.99905619
CCGGGAC3050.019.918128
CGGGACA3050.019.918129
CGGTTCA4750.019.4250456
AATGCCG4750.019.42013518
AGCGGTT4850.019.0245294
Produced by FastQC (version 0.11.5)