FastQCFastQC Report
Mon 6 Jun 2016
ERR1378828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912980
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACAAATCTCGTATGCCGTC13400.1467721089180486Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCACAAATCTCGTATGCC11230.12300378978728997Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG250.002107045635.9994345
ATGCCGT1400.035.35658644
TATGCCG1450.034.13739443
TGCCGTC1450.034.13739445
TCGTCGG450.001226474824.99960526
GCGGCCC450.001226474824.99960533
ATCGGAA2150.023.02289227
AGATCGG2250.021.99965125
GATCGGA2300.021.52139926
CCTTCGT955.5212695E-721.3189541
TCGGAAG2350.021.06349828
ACACCCG654.2377625E-420.76890212
CGCCACC1900.020.1334675
CTCGTAT2650.019.52799242
CGTATGC2700.019.16636544
TCTCGTA2750.018.81788441
CCGCCAC1202.4927976E-718.7517574
ATGCCGA5500.018.408819
TCGTATG2850.018.15760843
CTTCGTT1253.8273174E-718.0016882
Produced by FastQC (version 0.11.5)