FastQCFastQC Report
Mon 6 Jun 2016
ERR1378827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776629
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCCGTC15930.20511724388350164Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTAAGATCTCGTATGCC14000.1802662532560592Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1800.033.7474443
ATGCCGT1950.031.15148544
TGCCGTC2100.029.99772545
AGATCGG1601.8189894E-1221.0921525
CGTATGC2950.020.5916641
CTCGTAT2950.020.5916639
CGCCCGC908.218911E-620.0075021
TCGTATG3050.019.91652140
TCTCGTA3100.019.59528738
ATCTCGT3150.019.28425237
ACCGGCT3150.019.28425227
AGACCGG3200.018.98293525
CCGCCAC1202.4825567E-718.758244
TCGGAAG1801.0913936E-1118.74857728
ATCGGAA1801.0913936E-1118.74857727
GCGGTTC5200.018.6139475
AGCGGTT5200.018.6139474
GAGCGGT5200.018.6139473
AATGCCG5200.018.60435918
CCCGCCA1354.2453394E-818.341393
Produced by FastQC (version 0.11.5)