FastQCFastQC Report
Mon 6 Jun 2016
ERR1378823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187168
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTTAATCTCGTATGCCGTC15350.12929930725895578Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCGTTAATCTCGTATGCC12270.10335521173077442Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1750.037.28138443
TGCCGTC1900.034.3381245
ATGCCGT2000.032.62121244
TATATCG300.005150248329.9965226
CGTATGC3000.022.49738941
GATCGGA1104.0581654E-922.49738926
ATGCCGA4450.022.2446119
CCGTCGT2950.022.11607728
CGTCGTT2950.022.11607729
ACCGTCG3000.021.74747527
TGCCGAG4700.021.54005220
GTCGTTA3050.021.3909630
AATGCCG4700.021.06138418
CTCGTAT3150.020.7118839
GACCGTC3150.020.7118826
TCTCGTA3200.020.3882638
TCGTATG3200.020.3882640
CGGTTCA5000.020.2621576
AGACCGT3350.020.14691525
GCGGTTC5050.020.0615435
Produced by FastQC (version 0.11.5)