FastQCFastQC Report
Mon 6 Jun 2016
ERR1378820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265999
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTATGCCGTC16630.13135871355348622Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTATGCC13080.10331761715451591Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2200.038.8600944
TATGCCG2250.037.9965343
TGCCGTC2250.037.9965345
CGCAATT3800.023.09182229
AGACCGC3850.022.20664225
CTCGTAT3850.021.62225739
CCGCAAT4000.021.37473728
GACCGCA4000.021.37389426
TCGTATG4000.021.37389440
CGTATGC4100.020.85175541
ATCTCGT4000.020.81142237
CGCATGA550.003937035320.45429434
CGGAAGA3750.020.4102291
ACCGCAA4200.020.35689227
TCTCGTA4100.020.30382738
TCGTATA951.3170402E-518.9456399
CCCCGGA600.006511315218.7497734
CATCTCG4450.018.70689636
GCGGTTC6150.018.6642065
ATGCCGA6150.018.65683219
Produced by FastQC (version 0.11.5)