FastQCFastQC Report
Mon 6 Jun 2016
ERR1378819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948371
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTGTTATCTCGTATGCCGTC17710.18674126475820116Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTGTTATCTCGTATGCC12410.13085596248725448Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2450.038.56916843
TGCCGTC2550.037.05665245
ATGCCGT2700.034.9979544
CGAGTAT300.005148284629.99824119
ACCGGTT3600.026.24846327
TCGGAAG1900.026.05110728
CTCGTAT3650.025.88889539
TCTCGTA3650.025.88889538
GATCGGA1750.025.7127826
CGGTTGT3850.025.7127829
CGTATGC3700.025.53904241
GACCGGT3700.025.53904226
ATCTCGT3800.024.86827737
TATCTCG3800.024.86827736
AGACCGG3850.024.54401825
CCGGTTG3850.024.54401828
AGATCGG1850.024.32289725
TCGTATG3900.024.22935140
ATGCCGA5950.023.82213619
ATCGGAA1900.023.68282527
Produced by FastQC (version 0.11.5)