FastQCFastQC Report
Mon 6 Jun 2016
ERR1378818.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378818.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736548
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGGTTATCTCGTATGCCGTC11070.1502957037423223Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGGTTATCTCGTATGCC9400.12762236812807853Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1450.038.78795243
TGCCGTC1650.035.4498445
ATGCCGT1600.035.1515844
TTGCTCG500.002265590522.49701345
TCGGAAG1650.021.81676728
TCTCGTA2600.021.63321138
TCGTATG2600.021.63321140
ACCGTTG2600.021.63174227
CGTATGC2650.021.22359741
GATCGGA1700.021.17365826
GACCGTT2700.020.83056826
ATCTTCG654.218711E-420.7819925
AGATCGG1750.020.56869925
ATGCCGA4600.020.5407519
AATGCCG4500.020.49727818
TGCCGAG4500.020.49727820
TATCTCG2750.020.45321736
CCGTTGG2800.020.08798228
ATCTCGT2850.019.73556137
CTCGTAT2850.019.73556139
Produced by FastQC (version 0.11.5)