FastQCFastQC Report
Mon 6 Jun 2016
ERR1378817.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378817.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800796
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTACGTTATCTCGTATGCCGTC12340.1540966738095595Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACGTTATCTCGTATGCC11310.14123447170065784Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1850.036.48491743
ATGCCGT2150.031.39399744
TGCCGTC2150.031.39399745
CCGTACG2650.025.47060228
CGTACGT2700.024.99892429
ACCGTAC2700.024.99892427
GACCGTA2750.024.544426
CTCGTAT2850.024.47263139
TCTCGTA2800.024.10610638
TACGTTA2800.024.10610631
TCGTATG2850.023.68319140
GTACGTT2850.023.68319130
AGACCGT2950.022.88037325
CGTTATC2900.022.49903333
CGTATGC3050.022.13019641
TATCTCG2950.022.11769336
ATCTCGT3000.021.74906337
GAGACCG3000.021.74906324
CGGAAGA2300.021.5288771
GATCGGA955.5296914E-721.3148726
Produced by FastQC (version 0.11.5)