FastQCFastQC Report
Mon 6 Jun 2016
ERR1378816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572483
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAGTTATCTCGTATGCCGTC11130.19441625340839816Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCAGTTATCTCGTATGCC10480.1830622044672069Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG250.002107290335.99629630
TATGCCG1200.035.62133443
TGCCGTC1350.031.66340645
ATGCCGT1550.029.0292744
TATCTCG2150.023.02088739
CTCGTAT2200.022.49768442
TCGTATG2350.022.0190143
TCTCGTA2300.021.51952441
CGTCAGT2300.021.51952432
ATCTCGT2350.021.0616640
CCGTCAG2350.021.0616631
GAGACCG2350.021.0616627
ACCGTCA2350.021.0616630
GACCGTC2500.020.6978729
ACGCCCG550.003936507320.4524412
AGACCGT2550.020.2920328
CGTATGC2650.019.52629344
CGGCTAA851.2040498E-418.52750817
GTATGCC2700.018.33144645
CGAGACC2950.018.30320226
Produced by FastQC (version 0.11.5)