FastQCFastQC Report
Mon 6 Jun 2016
ERR1378814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966142
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCCGTC16260.16829824187334783Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTCTGATCTCGTATGCC9680.10019231127515417Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2200.039.88379745
TATGCCG2250.038.99748643
ATGCCGT2300.038.14971544
TGCGAGT301.1402191E-437.49758523
CGTATGC3450.025.43314441
CTCGTAT3450.025.43314439
TCGTATG3600.024.3734340
ATCTCGT3700.023.7146937
GACCGAT3700.023.7146926
TCTCGTA3700.023.7146938
CCGATTC3800.023.68268628
ACCGATT3850.022.7907427
AGACCGA3900.022.4985525
AATGCCG6000.021.748618
ATGCCGA6250.021.59860819
GAGACCG4100.021.40106224
AGATCGG1900.021.31441725
GCGGTTC6500.020.7764975
CGGTTCA6650.020.6463176
GTATGCC4250.020.6457342
Produced by FastQC (version 0.11.5)