FastQCFastQC Report
Mon 6 Jun 2016
ERR1378813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923054
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTCTTATCTCGTATGCCGTC15890.17214594162421698Illumina Paired End PCR Primer 2 (96% over 31bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTCTTATCTCGTATGCC11320.12263637880340694Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1900.040.2605945
TATGCCG1850.038.91643543
ATGCCGT2050.037.31469344
CTCGTAT2800.024.90912439
CGTATGC2950.024.40522241
TCTCGTA2900.024.0501938
TCGTATG2900.024.0501940
AGATCGG1900.023.682725
ATCGGAA1900.023.682727
CGAGTCT3050.023.60505129
AGACCGA3050.023.60505125
GAGACCG3100.023.22432524
GATCGGA1950.023.0754526
GACCGAG3150.022.85568426
ACCGAGT3150.022.85568427
AATGCCG5300.022.49856618
ATGCCGA5450.022.29215619
TATCTCG3200.021.79548536
TGCCGAG5500.021.68043320
ATCTCGT3250.021.4601737
Produced by FastQC (version 0.11.5)