FastQCFastQC Report
Mon 6 Jun 2016
ERR1378812.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378812.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920112
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCTTATCTCGTATGCCGTC18760.20388822230337175Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATCTTATCTCGTATGCC12670.13770062774966527Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3100.039.9167543
ATGCCGT3150.039.28315444
TGCCGTC3200.038.66935345
CTAGCGG250.002107565335.9976513
CTCGTAT4050.030.55356439
CCGGATC4200.029.46396628
TCTCGTA4200.029.46236438
ACCGGAT4150.029.275227
CGTATGC4250.029.11574741
TATCTCG4350.028.96362736
ATCTCGT4300.028.77719137
GACCGGA4250.028.5863726
GAGACCG4250.028.5863724
TCGTATG4450.028.31276140
AGACCGG4300.028.25397125
CGGATCT4500.027.49970229
GTATGCC4600.026.9004242
ATGCCGA6300.025.3554919
CCGAGAC4900.024.794322
TGCCGAG6500.024.5753220
Produced by FastQC (version 0.11.5)