FastQCFastQC Report
Mon 6 Jun 2016
ERR1378810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704170
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCCGTC25700.3649686865387619Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACCTTATCTCGTATGCC19040.270389252595254Illumina Paired End PCR Primer 2 (97% over 36bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT10560.14996378715367026Illumina Paired End PCR Primer 2 (96% over 30bp)
TTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGAC7070.10040189158867886Illumina Paired End PCR Primer 2 (100% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2500.037.7978545
ATGCCGT2850.034.73486744
TATGCCG2800.033.7480843
CAACGAG352.8190407E-432.1433126
TGAACCG300.00514734929.99829510
GGCGCCC406.165802E-428.12340211
AGGCGCC608.189269E-626.24850810
TCGTATA702.7022588E-522.49872210
CGTATGC4700.021.54132844
TATCTCG4700.021.06412939
ATCGGAA2150.020.9305327
TCGTATG4850.020.87510343
CTCGTAT4750.020.84092342
CCGAACC4550.020.76952628
CGAACCT4700.020.58539832
GACCGAA4700.020.58539829
ATCTCGT4850.020.41121140
TCTCGTA4900.020.20293441
ACCGAAC4800.020.15653830
AGACCGA4800.020.15653828
Produced by FastQC (version 0.11.5)