FastQCFastQC Report
Mon 6 Jun 2016
ERR1378809.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378809.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences824615
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACTGATCTCGTATGCCGTC14500.17583963425356075Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACTGATCTCGTATGCC12120.14697768049332113Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.038.71936443
ATGCCGT2250.036.99850544
TGCCGTC2400.034.68609645
CCGCGGC406.1661133E-428.12386319
CCGTTAC3650.022.80868128
CGTATGC3700.022.49909241
TCTCGTA3800.021.90833738
GAGACCG4000.021.37413624
ACCGTTA3900.021.34658427
CTCGTAT3950.021.07637639
GACCGTT3950.021.07637626
CGTTACT3950.021.07637629
AGACCGT3950.021.07637625
TCGTATG3950.021.07637640
CGGAAGA3100.021.0526431
CGAGACC4300.019.88291723
ATCTCGT4200.019.82182937
CCGAGAC4300.019.35968222
AAGAGCG5700.019.3460161
GTATGCC4350.019.13715742
Produced by FastQC (version 0.11.5)