FastQCFastQC Report
Mon 6 Jun 2016
ERR1378807.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378807.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730171
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTATCATCTCGTATGCCGTC10200.13969330471903157Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTATCATCTCGTATGCC9720.13311950214401833Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG708.1308826E-1032.13997343
ATGCCGT801.0004442E-1030.93472344
ACGGAAC300.005148303729.99730742
TGCCGTC751.7025741E-929.99730745
CTCGTAT2000.029.24737442
CCGGTTA1950.028.8455431
CGGTTAT2000.028.124432
GACCGGT2000.028.124429
TCGTATG2050.027.43656243
CGGAAGA2250.027.0105251
CGTATGC2150.026.16044244
TCTCGTA2150.026.16044241
ACCGGTT2200.025.56763630
AGACCGG2200.025.56763628
CATCTCG2300.024.45639
ATCTCGT2350.023.9356640
CCGAGAC2350.023.9356625
GAGACCG2450.022.95869627
GTATGCC2650.021.2245145
CGACCTC855.011112E-621.1847253
Produced by FastQC (version 0.11.5)