FastQCFastQC Report
Mon 6 Jun 2016
ERR1378806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672759
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTATGATCTCGTATGCC12560.18669389781481927Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTTATGATCTCGTATGCCGTC12510.1859506896228813Illumina Paired End PCR Primer 2 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC250.0021065435.9998213
TATGCCG1700.031.76218843
TGCCGTC1700.031.76218845
ATGCCGT1750.030.85469844
GTCGGAA300.005145883729.99985127
GACCGCT2500.020.69989826
CCGCTTA2500.020.69989828
CGCTTAT2550.020.29401829
CGTATGC2700.019.999941
TCTCGTA2700.019.999938
AGACCGC2600.019.9037525
ATGCCGA4200.019.2856219
CTCGTAT2850.018.94727339
CATCTCG600.00650235818.7526956
CGTGGCT600.006510734618.74851443
ACCGCTT2800.018.4820527
GAGACCG2800.018.4820524
GTATGCC3200.018.28115842
AATGCCG4450.018.20215818
ATCTCGT3000.017.9999137
Produced by FastQC (version 0.11.5)