FastQCFastQC Report
Mon 6 Jun 2016
ERR1378804.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378804.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186089
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGCTATTATCTCGTATGCCGTC15490.13059728232872914Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2100.034.2835643
ATGCCGT2250.031.99799344
GCGGTAC300.005148903529.99811717
CAACCGG300.005148903529.99811739
CGGTACT300.005148903529.99811718
TGCCGTC2650.028.86611445
ACGCCCG507.161648E-526.99830412
CTCGTAT2700.026.66499339
TCTCGTA2850.026.05099738
ACCGCGC709.3096423E-725.7137559
TCGTATG2800.025.71267140
CGGCTAT2850.025.26263829
CGTATGC2850.025.26157241
GACCGGC2850.025.26157226
TATCTCG2900.024.82602936
ACCGGCT3000.023.99849327
ATGCCGA4600.023.96588719
ATCTCGT3050.023.60507637
AGACCGG3050.023.60507625
AATGCCG4700.023.45597518
Produced by FastQC (version 0.11.5)