FastQCFastQC Report
Mon 6 Jun 2016
ERR1378803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647564
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGATTATCTCGTATGCCGTC17910.2765749794614895Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTGATTATCTCGTATGCC15700.24244707858991546Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT7590.11720849213359605Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC207.032549E-444.99799318
TATGCCG1800.037.4983343
TGCCGTC1950.035.76763545
ATGCCGT1900.035.5247344
AGGCGTT300.00514674129.99866139
TTCCGAA450.001224088225.006614
TATCTCG3550.023.44965739
TCTCGTA3650.023.42361341
ATCTCGT3650.022.80720340
CGTATGC3700.022.49899744
CTCGTAT3950.021.64460442
TCGTATG3850.021.62241243
CGGAAGA3750.021.6073781
CGCTGAT3900.021.34520132
ACCGCTG3950.021.07500830
AGACCGC4000.020.81157128
GATCGGA1850.020.67475318
TCGGAAG1850.020.67475320
GACCGCT4050.020.55463829
GTATGCC4050.020.55463845
Produced by FastQC (version 0.11.5)