FastQCFastQC Report
Mon 6 Jun 2016
ERR1378800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906534
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCATTATCTCGTATGCCGTC11130.12277531785901026Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCATTATCTCGTATGCC10380.11450204846150283Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1500.041.9958343
TGCCGTC1850.031.61848345
ATGCCGT1950.029.99702344
CTCGTAT2650.022.07327839
TCGTATG2700.021.66451640
ATGCCGA4000.021.37287719
CGTATGC2750.021.27061541
TATCTCG2900.020.94619636
TCTCGTA2800.020.89078338
CGTGCAT2850.020.52427729
GACCGTG2900.020.17041226
CCGTGCA2900.020.17041228
GCGGTTC4250.020.1278635
GATCGGA1251.7680577E-819.79803526
AATGCCG4350.019.65322118
ATCTCGT3000.019.49806437
AGACCGT3000.019.49806425
CCGAGAC3000.019.49806422
AGCGGTT4550.019.2955074
GAGCGGT4450.019.2264233
Produced by FastQC (version 0.11.5)