FastQCFastQC Report
Mon 6 Jun 2016
ERR1378796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684989
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTGTATCTCGTATGCCGTC18080.10730040374150811Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGTTGTATCTCGTATGCC17010.10095021391831045Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2650.039.0539543
ATGCCGT2950.035.0823644
TGCCGTC3200.034.45078345
TCGGAAG1550.026.12880728
TGCCGAG5750.025.04177720
CGCTTAG450.00122727824.998325
ATGCCGA5850.024.61371219
AATGCCG5950.024.20003518
TCTCGTA4300.024.06813238
ATCGGAA1700.023.82261727
CTCGTAT4350.023.79148739
GATCGGA1750.023.14128526
CGTATGC4500.022.99843841
GACCGTG4650.022.7403926
GAGACCG4650.022.7403924
GCGGTTC6650.022.3372655
TATCTCG4650.022.25655436
AGACCGT4650.022.25655425
TCGTATG4650.022.25655440
ATCTCGT4850.021.8026437
Produced by FastQC (version 0.11.5)