FastQCFastQC Report
Mon 6 Jun 2016
ERR1378795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649475
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCCGTC16300.2509719388737057Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTGTGTATCTCGTATGCC13100.20170137418684322Illumina Paired End PCR Primer 2 (97% over 35bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAAT8020.12348435274644905Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2050.039.51043743
ATGCCGT2100.038.5697144
TGCCGTC2150.037.6727445
AACGTAA450.001226298224.99888813
CGTATGC3350.024.17802841
CCGTTGT3350.024.17802828
TCTCGTA3450.023.47721738
TCGTATG3450.023.47721740
GACCGTT3600.023.12397226
ATCTCGT3550.022.81588737
CTCGTAT3550.022.81588739
CGTTGTG3600.022.49899929
AGACCGT3750.022.1990125
GAGACCG3650.022.19079424
TATCTCG3650.022.19079436
ACCGTTG3650.022.19079427
GCGGTTC5650.021.1101325
ATGCCGA5300.020.8009619
TGCCGAG5300.020.8009620
GTATGCC3950.020.50541742
Produced by FastQC (version 0.11.5)