FastQCFastQC Report
Mon 6 Jun 2016
ERR1378793.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378793.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences981318
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACGTATCTCGTATGCCGTC16840.17160594221241227Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTACGTATCTCGTATGCC10160.10353422641793995Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1100.038.8599443
TGCCGTC1300.031.15088345
ATGCCGT1350.029.99714544
CACCCGG952.1156666E-823.68316513
CACCGCT500.00226562822.497868
CGTTACG2050.021.95136829
GACCGTT2100.021.42653526
ACCCGGC955.5323835E-721.3148514
CCGTTAC2150.020.93037428
ACCGTTA2150.020.92930827
CGTATGC2150.020.92824241
GATCGGA1301.2278178E-920.76725426
CTCGTAT2100.020.35728339
ACGTATC2100.020.35728333
TATCTCG2150.019.88385636
GTTACGT2300.019.56535130
TCTCGTA2200.019.43195238
TTACGTA2250.019.0001331
TCGTATG2250.018.99916340
GTATGCC2750.018.81639142
Produced by FastQC (version 0.11.5)