FastQCFastQC Report
Mon 6 Jun 2016
ERR1378791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745606
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCCGTC16420.22022354970319447Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCAGTATCTCGTATGCC14960.20064216221435988Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1600.037.96558444
TGCCGTC1700.035.7323145
TATGCCG1650.035.45158443
AGTGCGT300.00514652529.9995129
AGATCGG1350.026.6644425
GATCGGA1400.025.7121426
TTCGTTG1550.023.2363343
TCGTTGG1550.023.2347764
TCGGAAG1650.023.18143828
ATCGGAA1650.021.8163627
CGTATGC2750.021.27095241
AATGCCG4550.021.26196318
ATGCCGA4550.021.26196319
CGTTGGA1700.021.1846475
GACCGTT2800.020.89251329
CGGGATT1201.1046723E-820.6232814
TGCCGAG4800.020.15456820
TATCTCG3050.019.9163736
CCGTTCA2950.019.83018528
CTCGTAT2950.019.82885439
Produced by FastQC (version 0.11.5)