FastQCFastQC Report
Mon 6 Jun 2016
ERR1378789.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378789.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886543
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTCTATCTCGTATGCCGTC16310.18397302781703764Illumina Paired End PCR Primer 2 (97% over 35bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAACTCTATCTCGTATGCC13480.15205128234050688Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2150.042.9047743
ATGCCGT2400.038.43552844
TGCCGTC2400.038.43552845
CGTAATA352.820711E-432.14120517
ACCGAAC4050.022.77660827
CTCGTAT4100.022.49884239
TATCTCG4100.022.49884236
CGTATGC4150.022.22777441
GAGACCG4150.022.22777424
TCGTATG4150.022.22777440
GACCGAA4200.021.96315626
AGACCGA4200.021.96315625
CGGAAGA3700.021.9018841
TCTCGTA4300.021.45238538
ATCTCGT4350.021.20580737
CCGAACT4350.021.20580728
AATTCCG550.003937010720.45349520
GTATGCC4550.020.27368542
ATGCCGA6000.019.87397819
AATGCCG6150.019.38924618
Produced by FastQC (version 0.11.5)