FastQCFastQC Report
Mon 6 Jun 2016
ERR1378788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731363
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATCTATCTCGTATGCC12340.16872606352796082Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATCTATCTCGTATGCCGTC11800.161342589111016Illumina Paired End PCR Primer 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1800.039.99562544
TATGCCG1850.038.9146643
TGCCGTC1900.037.8905945
CGGAAGA2600.024.2430361
GACTCCG602.4590213E-422.509853
GACGTTC500.002264367622.49907937
CCGCAAT3450.022.17300428
CTCGTAT3350.022.1632739
TATCTCG3300.021.81728636
TCTCGTA3450.021.52085738
CGCAATC3450.021.52085729
TCGTATG3350.021.49165740
CGTATGC3350.021.49018741
GCGGTTC4300.020.9365335
GACCGCA3450.020.8687126
AGACCGC3450.020.8687125
AGCGCTC654.2295567E-420.7740617
GAGACCG3500.020.57058524
TGCCGAG4300.020.40474520
ATCTCGT3550.020.28085937
Produced by FastQC (version 0.11.5)