FastQCFastQC Report
Mon 6 Jun 2016
ERR1378786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1378786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817773
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTACTATCTCGTATGCCGTC12850.15713407021263848Illumina Paired End PCR Primer 2 (96% over 32bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTTACTATCTCGTATGCC9450.11555774035092868Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1600.037.96529843
TGCCGTC1650.036.8148345
ATGCCGT1950.031.15101244
GACCGGT2650.022.92384126
AGACCGG2900.021.72348825
ACCGGTT2800.021.6957827
CCGGTTA2800.021.6957828
CGTATGC2800.021.69445441
CGGTTAC2850.021.31515129
CTCGTAT2900.020.94764939
TCTCGTA2900.020.94764938
TATCTCG2950.020.59260436
CGGAAGA2750.020.4639451
TCGTATG3050.019.9162240
GTATGCC3200.019.68570942
CCGAGAC3100.019.5949922
GAGACCG3400.018.52885824
TGCCGAG4150.018.43205620
ATCTCGT3300.018.4085437
GCGGTTC4050.018.3395145
Produced by FastQC (version 0.11.5)